one more thing I'm not going to be
it's boring as hell.
plus you have to actally keep a log book.
gross.
so my entire day was spent purifying less than 8 grams of protein.
so what I did yesterday was prepare for today. actually, I don't really know what I did yesterday except that it took a lot of running up and down stairs and lots of tiny amounts of stuff. and a protein gel.
so today I started with some solution of cells. minus the plasma membrane. so I guess I had one big cell. but without a plasma membrane. so actually I have no idea what I had. so first you add in some solution called PEI ( have no idea what it is or what it does.... I think she told me it precipitates the lipids out), then stir it for 1 hour, then add in ammonium sulfate (to precipitate something out.... forgot what. maybe this was the lipid.), then stir the mixture for 40min-1 hour, then balance, then centrifuge for 30 min, keep the supernatant and throw away the pellet, then add in more ammonium sulfate, then stir for 50 min, then centrifuge for 1 hour, and then throw away the supernatant and the pellet's the protein you want.
and in between all that while I was waiting for the damn centifuge I had to clean. =( it was a really dirty lab.
and I swear no one talks in that place. okay, there's not much to talk about.
but I did finish cat's cradle.
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