one more thing I'm not going to be
aka a scientist.
it's boring as hell.
plus you have to actally keep a log book.
gross.
so my entire day was spent purifying less than 8 grams of protein.
so what I did yesterday was prepare for today. actually, I don't really know what I did yesterday except that it took a lot of running up and down stairs and lots of tiny amounts of stuff. and a protein gel.
so today I started with some solution of cells. minus the plasma membrane. so I guess I had one big cell. but without a plasma membrane. so actually I have no idea what I had. so first you add in some solution called PEI ( have no idea what it is or what it does.... I think she told me it precipitates the lipids out), then stir it for 1 hour, then add in ammonium sulfate (to precipitate something out.... forgot what. maybe this was the lipid.), then stir the mixture for 40min-1 hour, then balance, then centrifuge for 30 min, keep the supernatant and throw away the pellet, then add in more ammonium sulfate, then stir for 50 min, then centrifuge for 1 hour, and then throw away the supernatant and the pellet's the protein you want.
and in between all that while I was waiting for the damn centifuge I had to clean. =( it was a really dirty lab.
and I swear no one talks in that place. okay, there's not much to talk about.
but I did finish cat's cradle.
it's boring as hell.
plus you have to actally keep a log book.
gross.
so my entire day was spent purifying less than 8 grams of protein.
so what I did yesterday was prepare for today. actually, I don't really know what I did yesterday except that it took a lot of running up and down stairs and lots of tiny amounts of stuff. and a protein gel.
so today I started with some solution of cells. minus the plasma membrane. so I guess I had one big cell. but without a plasma membrane. so actually I have no idea what I had. so first you add in some solution called PEI ( have no idea what it is or what it does.... I think she told me it precipitates the lipids out), then stir it for 1 hour, then add in ammonium sulfate (to precipitate something out.... forgot what. maybe this was the lipid.), then stir the mixture for 40min-1 hour, then balance, then centrifuge for 30 min, keep the supernatant and throw away the pellet, then add in more ammonium sulfate, then stir for 50 min, then centrifuge for 1 hour, and then throw away the supernatant and the pellet's the protein you want.
and in between all that while I was waiting for the damn centifuge I had to clean. =( it was a really dirty lab.
and I swear no one talks in that place. okay, there's not much to talk about.
but I did finish cat's cradle.
posted by sunshine at 8:35 PM
3 Comments:
bleh, and i get to get stuck with 8 weeks of solid biology stuff... joy
when my friends get to mess with all kinds of crap like explosives -__-
EXPLOSIVES... boo
hopefully it's not as interesting as it sounds
at least you can experiment with... different... cleaning techniques? 0_o
wow explosives...
love love love. omg you know the weirdest thing that happened to me. i was hearing "love, love, love" in my head. and i was like WHERE IS THAT FROM???
and then. i remembered that i wanted to dl the songs ryan sang at mr. plano. and i found out they were both beatles songs. and so i limewired beatles. and downloaded like the first 5 songs that came up. and then one of them was "all you need is love." and i listened to it and i was like HOLY CRAP. and then i was like...how do i even know this song. and then the trumpet part started and i was like oh yeah, love actually! that sure was some odd twist of cosmic fate.
holy crap those are lyrics from a backstreet boys song -___- wtf
Post a Comment
<< Home